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Proteomes of species with completed genomes (bacterial genomes, some eukaryotic genomes, human proteome - NCBI's most recent release - 01/2004) are
presented in the database. Sequences can be retrieved for analysis tools pertinent to two-dimensional gel-electrophoresis.
Sequences can be retrieved by:
description,
sequence fragment, or
molecular weight/ iso-electric point of the protein.
This interface represents an easy way of saving sequences in the for later use.
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2DE gel patterns are deposited by registered users.
The owner of the 2DE gel pattern can profile the gel (MW and pI profile),
and assign an open reading frame from the database to a given spot if the identity of the spot has been determined.
The image database contains not only scanned images, but also modeled gel patterns representing
a collection of images (e.g. a master pattern for a sample).
2DE gel patterns are grouped by:
tissue type,
sample type,
staining method used,
separation technique used in the first dimension (by charge),
the pH-range of the media used in first dimension,
technique used in the second dimension (by size).
2DE gel-patterns can be retrieved by:
species and
complex criteria from the database.
This interface represents an easy way of saving 2DE gel-patterns in the for later use.
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Tools pertinent to the querying of two-dimensional gel-electrophoresis are implemented and integrated into database.
When searching for sequences, tools that allow allow the discovery of sequences and alignment of
multiple sequences are presented.
Individual 2DE gel-patterns can be displayed or a collection of patterns can be animated.
The following tools are incorporated:
gel-display,
2DE animation,
ClustalW.
This interface represents an easy way of saving 2DE gel pattern-animations in the for later use.
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The main goal of is to incorporate 2DE gel patterns
from around the world. 2DE gel patterns representing proteins isolated from species with completed
genomes are accepted.
The common interface and characterization of 2DE gel patterns allows easy comparison.
Links to the same proteome database provides integration of all gels.
The following steps are required:
Registration (a valid e-mail address is used as a username)
Addition of tissue type, sample type, staining method, etc. if not in the database yet
Uploading of 2DE gel-pattern with the associated attributes
Profiling the MW and pI dimensions of the gel
Association of ORF(s) with a given spot on the gel
The content of bio-bag for users registered with is permanent.
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A variety of results from search engines can be added to the bio-bag;
a protein sequence, a 2DE pattern, a gel-animation, etc.
A checkbox is presented for each item in the result set provided by all the search engines.
After selecting the desired item(s), the item(s) can be added to the bio-bag by clicking on the
image.
These objects can be combined to create new animations, multiple sequence alignments, etc.
The content of bio-bag for users registered with is permanent.
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